Reporter

Part:BBa_K199043:Design

Designed by: Kin Lau   Group: iGEM09_MoWestern_Davidson   (2009-07-18)


pOmpC-RBS-Tet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 415
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 441
    Illegal NgoMIV site found at 809
    Illegal NgoMIV site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to plate ligation on a plate of TY media (low osmolarity) for colonies to grow


Source

Ligation of existing parts

References